Senior Scientist, PSC Biology (Genetics)

BlueRock Therapeutics
United States, Massachusetts, Cambridge
238 Main Street (Show on map)
Feb 12, 2025
The Senior Scientist, PSC Biology will be a strategic and technical leader of a team responsible for developing, testing, and implementing novel NGS (epi-) genome sequencing technologies to characterize human pluripotent stem cells (hPSCs) and their differentiated progeny at the molecular, (epi-) genomic level. This includes the use of NGS technologies to evaluate on- and off-target gene editing analysis of genome engineered PSCs. They will work independently and collaboratively participate in efforts to generate and analyze different types of sequencing data to gain further knowledge of PSC pluripotent state and their differentiation potential. They will also support and guide colleagues and their activities on the team, consistent with team leadership aligned program goals. Hands-on wet-lab experience with NGS sequencing technologies (nucleic acid extractions, sequencing library preparation, etc.) as well as a strong theoretical background in (human) genetics is essential; strong background in stem cell biology is preferred. Experience with hPSC culture, CRISPR/Cas-based genome engineering, and bioinformatics is strongly preferred. The incumbent will work closely with several collaborating teams, including through roles representing the team in cross-functional settings. This is an outstanding opportunity for a talented individual to contribute to bringing a new class of medicines to patients and gain valuable career experience in the cell therapy space. Collaboration with a highly motivated, world-class team of scientists and engineers promises an exciting and engaging work environment for motivated candidates. Responsibilities: Design and lead diverse NGS sequencing projects (including, but not limited to long-read, short-read genomic sequencing, bulk RNA-seq, scRNA-seq). Prepare and follow standard operating procedures, batch records, development and technical reports, and other process documentation. Manage and work with direct report to execute molecular biology experiments (NGS library preparation, digital droplet PCR) and hPSC culture activities (maintenance of hPSCs and lineage differentiation). Analyze and maintain experimental data in electronic laboratory notebooks in a timely and accurate manner. Maintain inventory of required reagents for experiments. Organize and present scientific data to peers through internal and external platforms, including oral presentations, informal discussions, and written communications. Coordinate activities across team members and collaborating teams to ensure project advancement and success. Provide guidance and support to colleagues on the team, leveraging prior experience and scientific expertise. This position requires excellent communication skills, an ability to work independently and collaboratively, and occasional overtime on the weekend and after hours. Minimum Requirements: PhD. in Cell Biology, Molecular Biology, Neuroscience, Biomedical Engineering, or related discipline, with 5+ years (B.S. with 12+ years; M.S. with 11+ years) of full-time laboratory experiences in a biological science discipline. Prior full-time laboratory experiences specific to human pluripotent stem cell biology and/or molecular genetics are strongly preferred. Knowledge in several of the following areas: genetics, molecular biology, stem cell biology, cell and developmental biology, regenerative medicine. Hands-on, wet lab experience with molecular biology/genetics techniques (PCR, NGS library prep) is required. Experience with hPSC culture, CRISPR/Cas-based genome engineering and bioinformatic analysis is strongly preferred. #LI-AL1